Journal article
Optimized methods to image hepatic lipid droplets in zebrafish larvae
N Khan, TM Salmi, AP Karamalakis, AR Nair, KC Sadler, AG Cox
Dmm Disease Models and Mechanisms | COMPANY BIOLOGISTS LTD | Published : 2024
DOI: 10.1242/dmm.050786
Abstract
The optical transparency of zebrafish larvae enables visualization of subcellular structures in intact organs, and these vertebrates are widely used to study lipid biology and liver disease. Lipid droplet (LD) presence is a prevalent feature of healthy cells, but, under conditions such as nutrient excess, toxicant exposure or metabolic imbalance, LD accumulation in hepatocytes can be a harbinger of more severe forms of liver disease. We undertook a comprehensive analysis of approaches useful to investigate LD distribution and dynamics in physiological and pathological conditions in the liver of zebrafish larvae. This comparative analysis of the lipid dyes Oil Red O, Nile Red, LipidTox and Li..
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Awarded by University of Melbourne
Funding Acknowledgements
T.M.S. was supported by a Yayasan Dayadiri Scholarship and a University of Melbourne Research Scholarship. The New York University Abu Dhabi (NYUAD) Faculty Research Fund and the Sheikh Hamdan Bin Rashid Al Maktoum Award for Medical Sciences to K.C.S. supported this work. N.K. is an NYUAD Kawader Fellow. N.K. was supported by the Office of Undergraduate Research at NYUAD. A.G.C. is supported by a National Health and Medical Research Council Investigator Grant (GNT1176650), an Australian Research Council Discovery Project Grant (DP200102693) and the Peter MacCallum Cancer Centre (Ted and Lila Seehusen Foundation). Open Access funding provided by University of Melbourne. Deposited in PMC for immediate release.